The olive tree, although recognised for its relative tolerance to abiotic stress, remains vulnerable to drought episodes intensified by climate change, which threatens its productivity. The study uses an integrated approach combining morpho-physiological, biochemical and proteomic analyses to examine the effects of severe water stress on the physiological performance of olive plants at the 'young seedling' stage. The results show a significant reduction in the relative water content (RWC), a decrease in leaf biomass and an alteration in the morphological characteristics of the leaves. These changes are accompanied by inhibition of photosynthesis and the onset of oxidative stress, resulting in damage to cell membranes and a decline in the physiological performance of the olive plants. Overall, this study provides solid data on the mechanisms involved in the response of young olive plants to water stress.